T4 ligase uniprot
Webgp4 is a hexameric protein containing two functional domains: helicase domain and primase domain. The helicase domain unwinds double-stranded DNA to provide template for replication. WebPerfect for multiple displacement amplification High fidelity - highly accurate replication Extreme processivity - capable of generating large fragments Isothermal amplification - no need for a thermocycler Reagents Supplied Need a custom/large volume order? Contact Us
T4 ligase uniprot
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WebT4 DNA Polymerase is supplied in a buffer of 200 mM potassium phosphate (pH 6.5), 10 mM DTT and 50% glycerol. More Information Applications Generation of blunt double-stranded DNA from DNA containing 5'/3' overhangs 5'-end or 3'-end labeling of double-stranded DNA In vitro mutagenesis Buffer WebDNA ligase, which is expressed in the early stage of lytic development, has been implicated in T4 DNA synthesis and genetic recombination. It may also play a role in T4 DNA repair. …
WebDNA ligase UniProt ID P00970 - DNLI_BPT4 Species Enterobacteria phage T4 (Bacteriophage T4) Gene 30 View Product On Supplier's Website Supplier provided … WebT4 DNA ligase is an enzyme that fixes broken DNA and seals it – similar to super glue. This particular DNA ligase was isolated from bacteriophage T4. During DNA replication or recombination, a break or a ‘nick’ in the backbone of DNA frequently occurs.
WebT4 RNA Ligase 1 catalyzes the ligation of a 5´ phosphoryl-terminated nucleic acid donor to a 3´ hydroxyl-terminated nucleic acid acceptor through the formation of a 3´ → 5´ phosphodiester bond with hydrolysis of ATP to AMP and PPi. Substrates include single-stranded RNA and DNA as well as dinucleoside pyrophosphates. Reagents Supplied WebT4 DNA Ligase from multiple suppliers was tested in reactions containing a fluorescent labeled single stranded, double stranded blunt, 3’overhang or 5’ overhang containing oligonucleotides. The percent degradation by …
WebMar 1, 2011 · Plasmid pET16b-T4Dnl contains the gp30 gene which encodes for T4 DNA ligase. The target protein is fused with 10xHis tag to facilitate the purification and recovery. pET16b-T4Dnl was...
WebSep 14, 2012 · Applicability of in vitro biotinylated ubiquitin for evaluation of endogenous ubiquitin conjugation and analysis of ubiquitin-associated protein-protein interactions has been investigated. Incubation of rat brain mitochondria with biotinylated ubiquitin followed by affinity chromatography on avidin-agarose, intensive washing, tryptic digestion of … how to train dragon movieWebSubtilisin proteases, found in all organisms, are enzymes important in the post-translational steps of protein processing. In Leishmania major and L. donovani, this enzyme has been described as essential to their survival; however, few compounds that target subtilisin have been investigated for their potential as an antileishmanial drug. In this study, we first … how to train employees on sexual harassmentWebThe T4-induced DNA ligase is partic- ularly interesting because of its unusual mode of joining action. T4 DNA ligase, which catalyzes the repair of phosphodiester breaks in DNA.DNA and DNA. RNA hybrid duplexes, is able to join duplex DNA molecules by two mechanisms. The enzyme can utilize either duplex DNA molecules which have how to train eidetic memoryWebDec 2, 2024 · Canonical ligases, including bacteriophage T4 RNA ligase 1 or Saccharomyces cerevisiae tRNA ligase Trl1, are ATP-dependent enzymes that catalyze the formation of a 3′,5′-phosphodiester linkage between 3′-hydroxyl (3′-OH) and 5′-phosphate (5′-P) RNA termini ( Banerjee et al., 2024; El Omari et al., 2006; Greer et al., 1983; Gu et … how to train each muscle groupWebT4 RNA Ligase 1 catalyzes the ligation of a 5´ phosphoryl-terminated nucleic acid donor to a 3´ hydroxyl-terminated nucleic acid acceptor through the formation of a 3´ → 5´ … how to train dragon moviesWebUniProt P00971 · RLIG_BPT4 Protein RNA ligase 1 Gene 63 Status UniProtKB reviewed (Swiss-Prot) Organism Enterobacteria phage T4 (Bacteriophage T4) Amino acids 374 … how to train evs pokemon scarletWebNov 1, 2005 · Molecular Architecture and Ligand Recognition Determinants for T4 RNA Ligase. RNA ligase type 1 from bacteriophage T4 (Rnl1) is … how to train ethics in the workplace