Data acquisition was performed on a Helios time-of-flight mass cytometer (CyTOF) coupled to a Hyperion Imaging System (Fluidigm). Selected areas for ablation were larger than the actual area of interest to account for loss of overlapping areas among sections due to cumulative rotation. The selected area ablated per … See more This study complies with all relevant ethical regulations and was approved by the ethics committee of the Canton Zurich (PB_2016-00811). See more Antibody panels were designed using AirLab34. All antibodies (Supplementary Tables 2–4 and Extended Data Fig. 1b) were conjugated to metals using the MaxPar X8 Multimetal Labeling Kit (Fluidigm) according … See more We used archived material from the tissue biobank at the University Hospital Zurich. Regions of interest in different breast carcinoma samples … See more For generating a 3D IMC model, first a tissue punch from an FFPE sample was re-cast into an EPON silicon mold by first putting the sample into a container of molten paraffin for a … See more WebApr 12, 2024 · (F and G) Shown are UMAPs visualizing CD8 + T cell populations in CyTOF datasets for non–small cell lung cancer ... Shown are MILAN-derived representative immunofluorescent images of indicated immune and cancer cell markers in SKCM (A) (left) or GBM (B) (left) tissue sections. Digital representations of indicated anatomical (middle) …
Mass Cytometry Core Facility - UNC School of Medicine
WebFeb 3, 2024 · Multispectral Scanning and Image Acquisition. Multispectral images (MSI) were acquired using the latest Vectra Polaris imaging system from Akoya. ... BCL6 and GATA3 implying an important role for GATA3 for human Tfh cell development. Our Cytof analysis revealed that the majority of tonsillar GATA3+ Tfh cells express a Tfh2 … WebCyTOF® Software offers support for the imaging and cell suspension-based sample analysis to Hyperion™ Imaging System and Helios™ system users. The software has the built-in capability to switch sam... v7.1 August 08, … how does the mental capacity act protect
Hyperion Imaging System Standard BioTools
WebApr 1, 2024 · (F) Image obtained from a melanoma sample showing signal for CD4 (T helper cells), CD8a (cytotoxic T cells), and FoxP3 (T regs). (G) Image obtained from a … WebCytometry by time of flight, or CyTOF, is an application of mass cytometry used to quantify labeled targets on the surface and interior of single cells. CyTOF allows the … WebMar 5, 2024 · Here, we build upon CITE-seq and computational methods for the integration of single-cell omics data ( 13, 14) to identify and annotate cell populations in mIHC images (or, more broadly, in highly multiplexed cytometry datasets) based on single-cell gene expression data of the same tissue. photocopiable oxford university press pdf