Bin2 phosphorylation mutant

Author: lumd

August undefined, 2024

WebAug 19, 2014 · Contrary to our expectations, BIN2 did not phosphorylate S75 or S77. Site-directed mutagenesis and subsequent in vitro kinase assays, employing single mutants (substitutions to A), revealed that... WebThus, BIN2 is a good candidate to regulate PIF4 levels, as inactivation of this kinase by BL may result in the fastest-migrating form of this factor seen in BL-treated seedlings ( Fig. 1C ). A direct interaction of the full-length BIN2 protein and PIF4 was actually observed in yeast two-hybrid assays ( Fig. 2A ).

POLAR-guided signalling complex assembly and localization

WebDa Som Lee1,2 iD Tae Hyeon An2,3 iD Hyunmi Kim2,3 iD Eunsun Jung4 iD Gyeonghun Kim5 iD Seung Yeon Oh6 iD Jun Seok Kim7 iD Hye Jin Chun8 iD Jaeeun Jung9 iD Eun-Woo Lee2,3 iD Baek-Soo Han3,10 iD Dai Hoon Han11 iD Yong-Ho Lee8,12 iD Tae-Su Han3,4 iD Keun Hur13 iD Chul-Ho Lee3,14 iD Dae-Soo Kim9 iD Won Kon Kim2,3 iD Jun … WebApr 20, 2024 · In this study, we show that the GSK3 kinase BIN2 physically interacts with CO and the gain-of-function mutant bin2-1 displays late flowering phenotype through down-regulation of FT transcription. Genetic analyses show that BIN2 genetically acts upstream of CO in regulating flowering time. cinnabon cinnamon roll inspired cake

Two Putative BIN2 Substrates Are Nuclear Components of …

WebSep 6, 2009 · GST–BIN2 M115A is a BIN2 kinase-inactive mutant. (e) GST–BIN2 or GST–bin2-1 was first treated with BSU1–YFP immunoprecipitated from BR-treated (+ BL; 0.25 μM for 30 min) or untreated 35S ... WebDec 20, 2012 · Mutant MBP-mBZR1 protein containing bzr1–1Dmuta- tion alsophosphorylated GST-BIN2.Consistent fastermobility mBZR1-CFPproteins im-munoblot analysis transgenicplants, bzr1–1D mutation ap- peared vitro-phosphorylatedMBP-mBZR1 protein hadlittle effect unphosphorylatedBZR1 fusion protein (Fig. 2B), suggesting … WebJun 4, 2009 · BES1 phosphorylation activity in the bin2-3bil1bil2 mutant is likely catalyzed by other GSK3s. A, Li + treatment rescued the short hypocotyl phenotype of etiolated … diagnostic code for shortness of breath

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Integrated omics networks reveal the temporal signaling events of ...

WebMay 14, 2024 · We thus tested whether BIN2 mediates the phosphorylation of BZR1 on high sugar medium. It is reported that BIN2 interacts directly with BZR1 through a 12-amino … WebBIN2 (brassinosteroid-insensitive 2) kinase, a GSK3-like kinase functioning as a negative regulator in BR signaling, directly phosphorylated RAPTOR1B (regulatory-associated protein of TOR 1B), a substrate-recruiting subunit in the TOR complex, at a conserved serine residue within a typical BIN2 phosphorylation motif. cinnabon cinnamon roll coffee nutritionWebNov 11, 2024 · e, Western blot with the custom-made p-BIN2 antibody on BIN2-WT or BIN2-AA (T423A and S424A non-phosphorylable mutant)-expressing A2780 cells showed that the p-BIN2 antibody detected an expected ... diagnostic code for tsh

"" - Bin2 phosphorylation mutant

Bin2 phosphorylation mutant

Modulation of BIN2 kinase activity by HY5 controls …

WebAug 25, 2016 · The regulatory mechanisms by which BRs and other signals regulate BIN2 activity and stability remain unclear. Although in BR signaling it is reported that BIN2 can be regulated by dephosphorylation of the Y200 residue (), the functions of many other phosphorylation sites remain unknown.In addition, overexpression of Y200 … WebJan 10, 2024 · Bottom schematic shows BASL polarization requires MAPK-mediate protein phosphorylation and the plasma membrane-associated BRX proteins. Unknown regulators (orange) are anticipated to mediate BASL polarization. ... Genotyping results showed that these mutant plants are ... GSK3-like BIN2 kinases 12 and the BSL phosphatases 13, …

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WebApr 6, 2015 · AtSK21/BIN2 phosphorylation of ARF2 alleviates DNA binding and repressor activity of ARF2, ... Notably, mutant bin2-1 protein accumulates in greater quantities than wild-type AtSK21/BIN2 in plant cells (Peng et al., 2008). The BR biosynthetic inhibitor BRZ induces the accumulation of AtSK21/BIN2 in plant cells. WebJun 6, 2024 · Considering the BIN2 inhibitor bikinin greatly increased SA-induced gene expression (Figure 1 D–1F and Supplemental Figure 1 B–1D), we examined the functional involvement of BIN2 in SA signaling.The bin2-1 gain-of-function mutant displayed reduced sensitivity to SA in a bacterial growth assay (Figure 2 B). Similarly, the inhibitory effect of …

WebBIN2 functions in the cross-talk between auxin and brassinosteroid signaling pathways. BIN2 regulates root epidermal cell fate specification by phosphorylating EGL3 and TTG1. BIN2-mediated phosphorylation appears to promote BZR1 export from the nucleus. KIB1 interacts with BIN2 blocking its interaction with substrates and promotes BIN2 degradation. WebApr 19, 2002 · To test if BIN2 phosphorylates BES1 or mutant bes1 in vitro, the proteins were expressed in E. coli, purified with GST or MBP tags, respectively, and used in an in vitro phosphorylation assay. BIN2 efficiently phosphorylated BES1 or mutant bes1 to a greater extent than BIN2 autophosphorylation, consistent with 22 putative BIN2 sites in …

WebMay 25, 2024 · The disease symptoms caused by V. dahliae were more severe in the gain-of-function mutant bin2-1 seedlings compared with the control plants Col-0. The loss-of … WebBIN2-mediated phosphorylation of ARF7 and ARF19 suppresses their interaction with AUX/IAAs, and subsequently enhances the transcriptional activity to their target genes …

WebIt was shown that the BIN2-catalyzed phosphorylation of BSK3 promotes the interactions of BSK3 with the BR receptor BRI1 (Brassinosteroid-Insensitive1) ... elimination of GSK3-like kinases resulted in an increased ratio of SE/CC whereas overexpression of a gain-of-function mutant variant bin2-1 slightly decreased the SE/CC ratio, ...

Webmany other phosphorylation sites remain unknown. In addition, overexpression of Y200 phosphatase BSU1 only partially rescued the BRI1 mutant bri1-116 (6), indicating that other mechanisms may also be involved in the regulation of BIN2 activity. Fur-thermore, BRs can regulate BIN2 degradation mediated by the 26S proteasome by unknown mechanisms ... diagnostic code for whirlpool gold dishwasherWebOur genetic, biochemical, and physiological assays revealed that despite functional redundancy, BIN2 plays a dominant role among the three group II members in regulating … diagnostic code for weight lossWebAug 1, 2024 · BR-INSENSTIVE 2 (BIN2), a crucial negative regulator of brassinosteroid (BR) signaling, is induced by ABA and phosphorylates SnRK2s to enhance ABA signaling [5]. PP2Cs, such as ABI1 and ABI2, function as negative regulators of both BIN2 and SnRK2s, and thereby form a negative feedback regulatory loop in ABA signaling [12]. cinnabon cinnamon roll syrupWebMar 30, 2024 · The BIN2 gain-of-function mutant bin2-1 displays an enhanced photomorphogenic phenotype in both far-red and red lights. phyA-enhanced accumulation of BIN2 promotes the phosphorylation of BES1 in far-red light. BIN2 acts genetically downstream from PHYA to regulate photomorphogenesis under far-red light. cinnabon cinnamon rolls with heavy creamWebtion, BIN2 phosphorylation stabilizes MYBL2. Such dual role of ... in the mutant (Fig. S1A), as reported previously (45). We created double mutants of mybl2 with bri1-5, a weak loss-of-function allele of BR receptor BRI1 (47), and bes1-D, a gain-of-function mutant in the BR pathway (4). Although the single mutant mybl2 diagnostic codes 5319 through 5323WebApr 11, 2024 · Highlights. As a major environmental factor impacting the seasonal growth and geographical distribution of plants, temperature change significantly affects crop quality and productivity. Ethylene is a gaseous hormone with an important role in plant growth, development, and multiple stress responses, including heat and cold. Temperature stress ... diagnostic codes 6510 through 6514WebMar 21, 2024 · GeneCards Summary for BIN2 Gene. BIN2 (Bridging Integrator 2) is a Protein Coding gene. Among its related pathways are Innate Immune System and … diagnostic code for thoracic pain